| Forum: Hemozoin Images |
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| Topic: Isolated P. falciparum hemozoin |
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Posts: 122
Registered:
June 2003
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| Topic: Birefringent P. falciparum hemozoin |
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Posts: 122
Registered:
June 2003
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| Topic: Heme aggregates from Tick |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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| Topic: Heme aggregate in Tick |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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| Topic: Heme aggregate in tick |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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| Topic: Heme aggregate in Tick |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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| Topic: Distribution of P.falciparum hemozoin |
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Posts: 122
Registered:
June 2003
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| Topic: Scanning electron micrographs of hemozoin from P.falciparum |
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Posts: 122
Registered:
June 2003
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| Topic: Freeze-fracture preparation of P.falciparum hemozoin |
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Posts: 122
Registered:
June 2003
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| Forum: Hemozoin protocols |
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| Topic: FPIX biomineralization inhibition test |
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Posts: 1
Location:
french guyana
Registered:
July 2003
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FPIX biomineralization inhibition test
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Fri, 11 July 2003 10:19
 
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from:
DEHARO, E., GARCIA, R., OPORTO, P., SAUVAIN, M., GAUTRET, PH., GINSBURG H. (2002) A non-radiolabeled ferriprotoporphyrin IX biomineralization inhibition test (FBIT) for the high throughput screening of antimalarial compounds.
Experimental Parasitology. Volume 100, Number 4, pp. 252-256.
Procedure for normal non sterile flat bottom 96-well plate
The procedure for testing FPIX biomineralization inhibition consists of incubating in a normal non sterile flat bottom 96-well plate at 370C for 18-24 hrs a mixture containing:
50 µl of 0.5 mg/ml of haemin chloride (Sigma H 5533) freshly dissolved in dimethylsulphoxide (DMSO),
100 µl of 0.5 M sodium acetate buffer pH 4.4 (prepared according to Deutscher, 1990).
50 µl of drug solution or 50 µl of solvent (for control), final pH is 5-5.2.
It is important to respect the following order: first the haemin solution, second the buffer, third the solvent or the solution of drug.
After incubation (at 370C for 18-24 hrs), the plate is centrifuged at 1600g for 5 min.
The supernatant is then discarded by vigorously turning upside down the plate, twice.
The remaining pellet is resuspended with 200 µl of DMSO to remove unreacted haematin.
The plate is then centrifuged once again and the supernatant eliminated in the same condition.
The pellet, consisting of precipitate of ß-haematin, is dissolved in 200 µl of 0.1 M NaOH for direct (in the same plate) spectroscopic quantification at 405 nm with a micro-ELISA reader (Titertek Multiskan MCC/340).
The data are expressed as the percentage of inhibition of FPIX polymerisation calculated by the following equation:
% inhibition = 100 x [(O.D.control - O.D.drug)/(O.D.control)].
The controls used in the test are water, DMSO, methanol, ethanol 100%, ethanol/water (70/30)
All tests are performed three time in triplicate.
Buffer: Acetate Buffer
Stock Solutions
A: 0.2 M solution of acetic acid (1 l.55 ml in 1000 ml)
B: 0.2 M solution of sodium acetate (16.4g of C2H302Na or 27.2 g of C2H302Na - 3H20 in 1000 ml)
X ml of A + Y ml of B, diluted to a total of 100 ml:
X Y pH
46.3 3.7 3.6
44.0 6.0 3.8
41.0 9.0 4.0
36.8 13.2 4.2
30.5 19.5 4.4
25.5 24.5 4.6
14.8 35.2 5.0
10.5 39.5 5.2
8.8 41.2 5.4
4.8 45.2 5.6
[Updated on: Fri, 11 July 2003 10:21]
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| Topic: Hemozoin electrophoresis at pH 11.2 |
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Posts: 122
Registered:
June 2003
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Hemozoin electrophoresis at pH 11.2
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Thu, 11 March 2004 14:21
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electrophoresis of malaria pigment at pH 11.2
HZ is only soluble above pH 11 and a specific electrophoretic separation technique is needed. A 6% - 20% polyacryamide gradient gel was prepared with 150mM phosphate buffer, pH 11.2. The anode buffer was 500ml of 10mM phosphate buffer, pH 11.2, and the cathode buffer consisted of 500ml of anode buffer mixed with 500mg SDS.The sample buffer was 3%SDS and 8M urea in 150mM phosphate buffer, pH 11.2. 10ul of isolated pigment were mixed with 20ul of sample buffer and applied to the gel. The separation was done at a constant voltage of 90V for 4hr ( J. Pharmacol. Toxicol. Meth. 32, 1994, 25-30). Hydrogen, hydrophobic and ionic bonds are broken by reagents such as urea, SDS and mild alkaline buffer, but coordination polymers are relatively stable. A coordination polymer would produce many different bands after separation at pH 11.2.
[Updated on: Thu, 21 October 2004 04:15]
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| Topic: Imprints of endopeptidase activity |
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Posts: 122
Registered:
June 2003
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| Topic: Methods In Malaria Research 5th edition |
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Posts: 122
Registered:
June 2003
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| Topic: links to websites of interest |
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Posts: 122
Registered:
June 2003
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links to websites of interest
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Thu, 30 November 2006 07:06
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Biochemistry of Plasmodium on the web: http://sites.huji.ac.il/malaria/ (H.Ginsburg, Jerusalem) http://www.tulane.edu/~wiser/malaria (M.Wiser, Tulan)
General:
http://www.fightingmalaria.org/ (Africa fighting malaria) http://www.amanet-trust.org/ (African Malaria Network Trust)
http://www.amref.org/ (African Medical & Research Foundation)
http://www.appmg-malaria.org.uk/ (All-Party Parliamentary Malaria group, UK)
http://www.cdc.gov/ (Centers for Disease Control and Prevention)
http://www.cdc.gov/travel/malinfo.htm (CDC,Malaria, General Informations) http://www.nlm.nih.gov/archive/20040830/pubs/cbm/malaria.htm l (current bibliographies in medicine, malaria) http://www.ebi.ac.uk/parasites/parasite-genome.html (EMBL parasite genome database) http://www.theglobalfund.org (Global Fund to Fight AIDS, TB and Malaria)
http://impact-malaria.com/en/(Impact Malaria)
http://www.map.ox.ac.uk/MAP-intro.pdf (Malaria atlas project)
http://www.malariaconsortium.org/(Malaria Consortium) http://www.malaria.org/ (Malaria Foundation International
http://www.malariagen.net/ (Malaria Genomic Epidemiology Network)
http://www.malaria.org.za/ (Malaria in Southern Africa) http://www.malariajournal.com (Malaria Journal) http://www.hanmat.org/links.htm (Malaria links and malaria travel informations) http://www.malaria-reference.co.uk (Malaria Reference Laboratory, London)
http://www.anopheles.org (Malaria Research Portal)
http://www.nlm.nih.gov/mimcom(Malaria Research Resources) http://malaria.researchtoday.net/ (Malaria Research Today) http://www.mara.org.za/ (Malaria Risk in Africa) http://www.malariasite.com/ (Malaria Site)
http://www.malariavaccine.org](Malaria Vaccine Initiative) http://www.mmv.org/ (Medicines for Malaria Venture) http://www.masta.org/ (Medical Advisory Services for Travellers Abroad)
http://www.nlm.nih.gov/medlineplus/malaria.html (MedlinePlus: Malaria)
http://www.mim.su.se/ (MIM, Multilateral Initiative on Malaria) http://www.malaria.mr4.org/ (MR4 homepage)
http://www.fit-for-travel.de(München, Germany) http://www.nature.com/nature/malaria/index.html(Nature,Malaria Genome) http://www.rollbackmalaria.org (Roll Back Malaria) http://www.acgt.co.za/sami/ (South African Malaria Initiative, SAMI)
http://www.malaria.org.zw/index.html (Southern Africa Malaria Control)
http://www.ssi-tdr.net (South-South Initiative for tropical disease research)
http://sequence-www.Stanford.edu/group/malaria/index.html (Stanford genome project)
http://www.swissinfo.org/sen/Swissinfo.html?siteSect=670(Swissinfo's Malaria information website) http://www.sanger.ac.uk/Projects/P_falciparum (The Sanger Centre P.falciparum genome project) http://www.tigr.org/tdb/edb2/pfa1/htmls (TIGR P.falciparum genome database)
http://www.traveldoctor.co.uk/malaria.htm (Traveldoctor) http://mosquito.who.int (WHO Roll Back Malaria) http://www.who.int/tdr (WHO/TDR homepage) http://www.wehi.edu.au/MalDB-www/who.html (WHO/TDR Malaria Database)
Schools:
http://www.bni.uni-hamburg.de/ (BNI, Hamburg, Germany)
http://www.md.huji.ac.il/depts/parasitology (Hadassah, Jerusalem, Israel)
http://www.hsph.harvard.edu/malaria/contact.html (Harvard School, Boston, USA)
http://www.jhsph.edu/malaria (JHMRI, Baltimore, USA)
http://www.lambarene.org/ (Lambarene, Gabon)
http://www.liv.ac.uk/lstm/(LSTM,Liverpool,UK)
http://www.malaria-reference.co.uk (LSHTM,London,UK)
http://www.mrcindia.org/ (MRC, India)
http://www.nimr.mrc.ac.uk (NIMR, London, UK)
http://www.dbbm.fiocruz.br/ (Oswaldo Cruz, Rio de Janeiro, Brazil)
http://www.rph.wa.gov.au/malaria.html (Royal Perth Hospital, Perth, Australia)
http://www.shoklo-unit.com (Shoklo Malaria Research Unit, Thailand)
http://www.sanger.ac.uk/ ((The Sanger Centre)
http://wrair-www.army.mil/ (Walter Reed Army Institute of Research)
http://http://www.wehi.edu.au/ (WEHI, Melbourne, Australia)
http://www.wellcome.ac.uk/en/malaria/home.html (Wellcome Trust - Malaria)
[Updated on: Thu, 29 March 2007 14:38]
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| Forum: Hemozoin pdf's |
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| Topic: parasite-host interaction |
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Posts: 122
Registered:
June 2003
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| Topic: Article describing nem tool to study intracellular heme traffic. |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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| Topic: Article describing nem tool to study intracellular heme traffic. |
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Posts: 8
Location:
Bioquimica Médica, Brasil
Registered:
February 2004
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